Liquid chromatography |
- Mass spectrometry (LC-MS) |
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- HPLC with electrochemical detection (LCEC, |
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amperomety, coulometry) |
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- HPLC with fluorescence detection |
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- HPLC with UV/VIS (DAD) detection |
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- HPLC with conductivity detection |
Immunoassays |
- EIA, ELISA with UV/VIS, FL, TRF and LM detection |
Enzyme assays |
- Enzyme activity |
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- Biomarkers including glucose, lactate, pyruvate, |
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glycerol |
Spectrophotometric assays |
- Inorganic ions, calcium, magnesium, urea |
Method development and validation |
- LC-MS/MS for measuring unusual endogenous |
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molecules, drugs and metabolites |
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UHPLC-MS/MS and UPLC-TOF systems for small and large molecules Techniques applying ultra high performance liquid chromatography with tandem mass spectrometry and time-of-flight mass spectrometry allow detection of endogenous molecules, as well as, test compounds, drugs used as positive controls and their metabolites in biological samples including microdialysates, cell lysates, CSF, plasma and brain tissue extracts. |
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HPLC techniques with electrochemical or fluorescence detection optimized for analysis of neurotransmitters, neuromodulators and related molecules in the microdialysates Serotonin, noradrenaline, dopamine and their metabolites, acetylcholine, GABA, aspartate and glutamate, glycine, physiological amino acids, histamine, adenosine and other purines, cAMP, cGMP, kynurenic acid and other metabolites of the kynurenine pathway, radical oxygen species (ROS), NO2-/NO3-. |
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ELISA and enzymatic spectrophotometric assays Neuropeptides, hormones, A-beta and tau peptides, synuclein, cytokines, BDNF, NGF, interleukins enzymes and other clinical biomarkers. Lactate, pyruvate, glycerol, glucose, urea (CMA Microdialysis Analyser) for clinical microdialysates, Mg2+, Ca2+ and other ions.. |
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Ultra-sensitive detection of monoamines Unique fluorescence derivatization/HPLC methods developed for simultaneous determination of noradrenaline, dopamine and serotonin down to 10 -11 M [100 atmol/20 µl] and for histamine [300 atmol/20 µl]. |